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Dan Ohtan Wang, Ph.D.



2007               Summer Brain Science Institute, RIKEN summer course on Brain science: Mystery and Mission, Japan

2005               Summer Cold Spring Harbor Laboratory course on Biology of Memory

2002-2004     University of Southern California, Ph.D. Neuroscience

2000-2002     The Cellular and Molecular Biology graduate program, University of Alabama, Birmingham, USA

1998-2000      Tokyo Institute of Technology, Japan, M.A. Biosciences

1994-1998       Tokyo Institute of Technology, Japan, B.S. Bioengineering



Madugalle SU* Meyer K, Wang DO, and Bredy TW*. RNA N-Methyladenosine and the Regulation of RNA Localization and Function in the Brain. (2020) Trends Neurosci. (Link)


Wang DO*. RNA modifications in the central nervous system. Oxford Handbook on Neuronal Protein synthesis. (Link)


Roy R, Shiina N*, Wang DO*. More dynamic, more quantitative, unexpectedly intricate: advanced understanding on synaptic RNA localization in learning and memory. (2020) Neurobiol Learn and Mem. 168


Liu X, Gao Y, Long X, Hayashi T, Mizuno K, Hattori S, Fujisaki H, Ogura T, Wang DO, Ikejima T. Type I collagen promotes the migration and myogenic differentiation of C2C12 myoblasts via the release of interleukin-6 mediated by FAK/NF-κB p65 activation. (2020) Food & Function. 11. 328-338.



Wang DO*. Mapping m6A and m1A with mutational signatures. (2019) Nat Methods. DOI:10.1038/s41592-019-0636-z.


Liu X, Long X, Gao Y, Liu W, Hayashi T, Mizuno K, Hattori S, Fujisaki H, Ogura T, Onodeta S, Wang DO, Ikejima T. Type I collagen inhibits adipogenic differentiation via YAP activation in vitro. (2019) Journal Cell Physiol. 235(2),1821-1837


Kashida S, Wang DO, Saito H, Gueroui Z. Nanoparticle-based local translation reveals mRNA as translation-coupled scaffold with anchoring function. (2019) PNAS, 116(27), 13346-13351


Sukegawa M, Wang DO*. Neuropsychiatric disorders and RNA chemical marks at synapse. (2019) Journal of Clinical and Experimental Medicine (Japanese) Vol. 269 (11), p.811-812



Merkurjev D, Hong WT, Iida K, Goldie BJ, Yamaguti H, Oomoto I, Ohara T, Kawaguchi S, Hirano T, Martin KC, Pellegrini M, Wang DO*. Synaptic N6 methyladenosine (m6A) reveals functional partitioning of localized transcripts.  (2018) Nature Neuroscience, 21, 1004–1014


Niwa M, Hirayama T*, Oomoto I, Wang DO, Nagasawa H. Fe(II) ion release during endocytotic uptake of iron visualized by a membrane-anchoring Fe(II) fluorescent probe. (2018) ACS Chem. Biol. 13(7), 1853–1861


Wang DO. Live Imaging of Nuclear RNPs in Mammalian Complex Tissue with ECHO-liveFISH. (2018) Methods Mol Biol. 1649:259-272


Wang DO, Ninomiya K, Mori C, Koyama A Haan M, Kitabatake M, Hagiwara M, Chida K, Tahakashi S, Ohno M*, Kataoka N*. Transport granules bound with nuclear cap binding protein and exon junction complex imply a role of pioneer round translation in mRNP remodeling in human neuronal processes. (2017) Front. Mol Biosci


Goldie BJ, Fitzsimmons C, Weidenhofer J. Atkins JR, Wang DO, and Cairns MJ. miRNA enriched in human neuroblast nuclei bind the MAZ transcription factor and their precursors contain the MAZ consensus motif. (2017) Front. Mol Neurosci DOI: 10.3389/fnmol.2017.00259



Ohmachi M, Fujiwara Y, Muramatsu S, Yamada K, Iwata O, Suzuki K, Wang DO*. A modified single-cell electroporation method for molecule delivery into a motile  ustral, Euglena gracilis. (2016) J Microbiol Methods Nov;130:106-111. This work is highlighted in “Biomedial Advances”



Oomoto I, Wang DO. Visualization of ncRNA with fluorogenic hybridization oligonucleotide probes. (2015) Jikkenigaku (Japanese) Vol.33 No.20 3197-3447


Oomoto I, Hirano-Suzuki A, Umeshima H, Han YW, Yanagisawa H, Carlton P, Harada Y, Kengaku M, Okamoto A, Shimogori T, and Wang DO*. ECHO-liveFISH: in vivo RNA Labeling Reveals Dynamic Regulation of Nuclear RNA Foci in Living Tissues. (2015) Nucl Acids Res DOI:10.1093/narlgkv614  
*This work has been featured in “Nikkei Sangyo Shimbun” and “Weekly Economist”.


Hayashi G, Yanase M, Takeda K, Sakakibara D, Sakamoto R, Wang DO, Okamoto A*. Hybridization-sensitive fluorescent oligonucleotide probe conjugated with a bulky module for compartment-specific mRNA monitoring in a living cell. (2015) Bioconjug Chem. Mar 18;26(3):412-7. 


Wang, DO*, Okamoto. ECHO-FISH for Gene Transcript Detection in Neuronal and Other Cells and Subcellular Compartments” A. (2015) Neuromethods, 99 


Wang DO*, Okamoto A. Visualization of nucleic acids with synthetic exciton-controlled fluorescent oligonucleotide probes. (2015) Methods Mol Biol.; 1262:69-87



Wang DO*, Haan M. Effective in situ detection of nucleic acids with photochemical oligonucleotide probes. (2014) Lecture notes on cell biology and materials sciences.; 15-26


Sanghamitra. NJM, Inaba H, Arisaka F, Wang DO, Kanamaru S, Kitagawa S and Ueno T*. Plasma membrane translocation of a protein needle based on a triple-stranded β-helix motif. (2014) Mol. BioSyst 10(10):2677-83.



Sato S*, Watanabe M, Katsuda Y, Murata A, Wang DO, Uesugi M*. Live-Cell Imaging of Endogenous mRNAs with a Small Molecule. (2014) Angew Chem Int Ed Engl. Dec 23. DOI: 10.1002/anie.201410339.


Diring S, Wang DO, Kim C, Kondo M, Chen Y, Kitagawa S, Kamei K*, Furukawa S* Localized cell stimulation by nitric oxide using a photoactive porous coordination polymer platform. (2013) Nat Commun 4:2684 


Okamoto A*, Sugizaki K, Yuki M, Yanagisawa H, Ikeda S, Sueoka T, Hayashi G, Wang DO. A nucleic acid probe labeled with desmethyl thiazole orange: a new type of hybridization-sensitive fluorescent oligonucleotide for live-cell RNA imaging. (2013) Org Biomol Chem 11(2): 362-71



Wang DO and Akimitsu Okamoto*. ECHO probes: fluorescence emission control for nucleic acid imaging. (2012) Journal of Photochemistry and Photobiology, C: Photochemistry Reviews 13(2): 112-123


Meer E, Wang DO, Kim SM, Barr I, Guo F, and Martin KC. Identification of a cis-element that localizes mRNA to synapses. (2012) PNAS 109 (12): 4639-44 

(Equal contribution)


Wang DO*, Hitomi Matsuno, Shuji Ikeda, Hiroyuki Yanagisawa, Yasunori Hayashi, and Akimitsu Okamoto*. A quick and simple FISH protocol with hybridization-sensitive fluorescent linear oligodeoxynucleotide probes. (2012) RNA  Jan;18(1):166-75 (* Corresponding authors)



Okamoto A*, Ikeda S, Kubota T, Wang DO. Design of binary energy transfer on nucleic acids. (2011) Photomed. Photobiol 33, 45-46


Ikeda S, Kubota T, Wang DO, Yanagisawa H, Umemoto T, Okamoto A*. Design and Synthesis of Caged Fluorescent Nucleotides and Application to Live Cell RNA Imaging. (2011) ChemBioChem Dec 16;12(18):2871-80.


Ikeda S, Kubota T, Wang DO, Yanagisawa H, Yuki M, Okamoto A*. Emission Control by Binary Energy Transfer Processes on Oligouridine. (2011) Org. Biomol. Chem. Sep 9;9(19):6598-603.


Ikeda S, Yanagisawa Y, Nakamura A, Wang DO, Yuki M, Okamoto A*. Hybridization-sensitive fluorescence control in the near-infrared wavelength range. (2011) Org. Biomol. Chem. Jun 7;9(11):4199-204


Wang D, Matsuno H, Ikeda S, Hayashi Y, Okamoto A. A novel FISH method detecting single mismatches under 25 minutes. (2011) Neuroscience Research 71, E122,



Wang DO, Martin KC*, and Zukin RS*. Spatially restricting gene expression by local translation at synapses. (2010) Trends Neurosci. 33(4):173-82



Zhao Y, Wang DO, Martin KC*. Preparation of Aplysia sensory-motor neuronal cell cultures. (2009) J Vis Exp. Jun 8;(28).


Wang DO, Kim SM, Zhao Y, Hwang HG, Miura SK, Sossin WS, and Martin KC*. Synapse- and stimulus-specific local translation during long-term neuronal plasticity. (2009) Science. 324(5934): 1536-40.

* This work was highlighted in a “Perspectives” article by M. Korte in the same issue of Science, featured as an “Editor’s choice” in Sci. Signal., 23 June 2009, selected as a “must read” article by Faculty of 1000, and featured in popular science media including Fortune, Yahoo news and BBC Focus.



Lai KO, Wang D and Martin KC*. Signaling between synapse and nucleus during synaptic plasticity. (2008) Intracellular traffic and neurodegenerative orders, research and perspectives in Alzheimer’s disease, Springer-Verlag Berlin Heidelberg, pp71-85



Wang D, Hu J, Leitzell K, and Quick MW*. Neurotransmitter transporters. Sibley D, Hanin I, Kuhar M and Skolnich P (ed.) (2007) The Handbook of Contemporary Neuropharmacology, John Wiley and Sons, Inc., New York



Wang D and Quick MW*. Trafficking of the plasma membrane GABA transporter GAT1: Size and rates of an acutely recycling pool. (2005) J Biol Chem. 280:18703-18709.



Quick MW*, Hu J, Wang D, and Zhang HY. Regulation of a gamma –aminobutyric acid (GABA) transporter by reciprocal tyrosine and serine phosphorylation. (2004) J Biol Chem. 279:15961-15967.



Wang D, Deken SL, Whitworth TL and Quick MW*. Syntaxin 1A inhibits GABA flux, efflux, and exchange mediated by the rat brain GABA transporter GAT1. (2003) Mol Pharmacol. 64:905-913.


Quick MW, Wang D, Zhang H. GABA transporters: update on uptake. (2003) J of Neurochemistry. 87, 43


Deken SL, Wang D and Quick MW*. Plasma membrane GABA transporters reside on distinct vesicles and undergo rapid regulated recycling. (2003) J Neurosci. 23:1563-1568.



Deken SL, Wang D, and Quick MW*. Methods in studying the regulation and trafficking of transmembrane transporters. In M Quick (ed.) (2002) Transmembrane Transporters, John Wiley and Sons, Inc., New York, pp. 111-123.


Chiu CS, Jensen K, Sokolova I, Wang D, Li M, Deshpande P, Davidson N, Mody I, Quick MW, Quake SR and Lester HA*. Number, density, and surface/cytoplasmic distribution of GABA transporters at presynaptic structures of knock-in mice carrying GABA transporter subtype 1-green fluorescent protein fusions. (2002) J Neurosci. 22:10251-10266.



Sato H, Wang D and Kudo A*. Dissociation of Pax-5 from KI and KII sites during kappa-chain gene rearrangement correlates with its association with the underphosphorylated form of retinoblastoma. (2001) J Immunol. 166:6704-6710.


Wang D and Kudo A*. The regulation of Ig rearrangement in bone marrow and spleen. (1999) JikkenIgaku (Japanese). 17:16-20.

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